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Biotechnology: Genotyping of Pseudonwnas aeruginosa by Polymerase chain Reaction - based DNA fingerprinting

 

 

Genotyping of Pseudonwnas aeruginosa by Polymerase chain Reaction - based DNA fingerprinting

SAHAR A. AL-SHALCHI, * JALADET M.S. JUBRAEL ALICE K .MELCONIAN

Biotechnology Dept./ College of Science / Baghdad University * IPA Agricultural Research Center Key word : P. aeruginosa , Genotyping, RAPD, DNA finger printing

ABSTRACT

Pseadomonas aeruginosa is a major cause of nosocomial infections, usually in the context of serious underlying disease. Eighteen clinical isolates of P. aeruginosa collected from patients suffering from burn infections , in addition to two different hospital environmental samples were analyzed by Random Amplified Polymorphic DNA ( RAPD ).

The isolates tested formed nine different RAPD patterns when typed with highest discriminatory power primer OPN-16 , while using the primer OPG-O8 eight different genotypes were detected.

The results of this study support the visibility of using such markers for genotyping of P. aeruginosa in epidemiological studies as well as in the control of nosocomial infections.



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